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- Title
Levan Production by Paenibacillus polymyxa Immobilized on Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles Using Molasses Medium with Emphasis on the Bioactivity.
- Authors
Koşarsoy Ağçeli, Gözde; Günan Yücel, Hande
- Abstract
In this study, levan biopolymer (a fructose-based exopolysaccharide) was produced by Paenibacillus polymyxa HCT33-3, which was immobilized onto the composite of silica-coated magnetite nanoparticles (Fe3O4@SiO2 NPs). For this purpose, firstly, synthesis of magnetite nanoparticles (Fe3O4 NPs) was followed by Fe3O4@SiO2 composite formation to enhance its stability. The characterization studies were investigated with the scanning electron microscopy and energy dispersive X-ray analyzes, transmission electron microscopy, X-ray diffraction analyses. Then, the microorganism Paenibacillus polymyxa HCT33-3, immobilized onto Fe3O4@SiO2 and levan fermentation was carried out in the media including molasses. Effects of molasses percentage in the growth media, initial pH, temperature and fermentation period parameters were tested on levan production capability. The highest levan concentration of 35.8 g/L was obtained at the 54th hour in the medium including 20% molasses (v/v) with the initial pH and temperature values of 7.0 and 37 °C, respectively. The characterization studies clearly confirmed that the produced exopolysaccharide was levan, which showed an antimicrobial effect against all microorganisms used in the study, besides its effectiveness on the biofilm formed by Pseudomonas aeruginosa. This is the first study focusing on high-value levan biopolymer production by the immobilized microorganisms onto Fe3O4@SiO2 nanocomposite in the growth media including molasses as the sole carbon source. This environmentally friendly process, which can potentially enable the repeated use of the cells, seemed to be significantly advantageous in terms of both cost and sustainability.
- Subjects
MICROBIAL exopolysaccharides; PAENIBACILLUS; MOLASSES; NANOPARTICLES; TRANSMISSION electron microscopy; SCANNING electron microscopy
- Publication
Journal of Polymers & the Environment, 2023, Vol 31, Issue 12, p5355
- ISSN
1566-2543
- Publication type
Article
- DOI
10.1007/s10924-023-02960-5