We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Neutron Macromolecular Crystallography for Biological Samples—Current State and Future Perspectives.
- Authors
Hjorth-Jensen, Samuel John; Budayova-Spano, Monika
- Abstract
Knowledge of hydrogen locations and protonation states is critical for a fundamental understanding of biological macromolecular function/interactions, and neutron macromolecular crystallography (NMX) is uniquely suited among the experimental structural-determination methods to provide this information. However, despite its potential, NMX remains a relatively niche technique, due to substantial limitations. This review explores NMX's role amongst the evolving landscape of structural biology, comparing and contrasting it to the historical gold standard of X-ray macromolecular crystallography (X-ray MX) and the increasingly prevalent electron-based methods—i.e., electron microscopy (EM) and electron diffraction (ED). Forthcoming developments (e.g., the European Spallation Source in Lund, Sweden, coming online) are expected to substantially address current limitations and ensure NMX will remain relevant in the coming decades.
- Subjects
LUND (Sweden); CRYSTALLOGRAPHY; X-ray crystallography; NEUTRONS; ELECTRON diffraction; ELECTRON microscopy; SMALL-angle X-ray scattering
- Publication
Crystals (2073-4352), 2024, Vol 14, Issue 5, p433
- ISSN
2073-4352
- Publication type
Article
- DOI
10.3390/cryst14050433