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- Title
High resolution melting analysis of DNA microsatellites in olive pastes and virgin olive oils obtained by talc addition.
- Authors
Pasqualone, Antonella; Rienzo, Valentina Di; Miazzi, Monica Marilena; Fanelli, Valentina; Caponio, Francesco; Montemurro, Cinzia
- Abstract
Talc (hydrated magnesium silicate) is a physical coadjuvant that can be employed in the production of extra virgin olive oil to increase yield. The adsorbent properties of talc could hamper DNA recovery, leading to false negatives in DNA analysis. The aim of this work was to verify the effect of talc addition on olive oil DNA by targeting four selected microsatellites. Olive processing trials were carried out at two different levels of talc (1 and 2%) and without talc (control). DNA extraction yield and purity level were ascertained, and High Resolution Melting (HRM) analysis of microsatellites was subsequently applied to the extracted DNA. Neither the DNA extraction yield nor the A260/230 and A260/280 ratios showed significant differences between control and talc-treated samples. Higher values of A260/230 and lower yields were observed in olive oils than in olive pastes. The DNA microsatellites analyzed showed identical HRM profiles in all the samples, excluding any effect of talc and confirming the genetic homogeneity of the olive lot processed (cv. Coratina).
- Subjects
OLIVE oil; MICROSATELLITE repeats in plants; MELTING; FRUIT pastes; PLANT DNA
- Publication
European Journal of Lipid Science & Technology, 2015, Vol 117, Issue 12, p2044
- ISSN
1438-7697
- Publication type
Article
- DOI
10.1002/ejlt.201400654