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- Title
Molecular Prevalence, Hematological Biomarker, Associated Risk Factors and Chemotherapeutic Trials of Ehrlichiosis in Dogs in Pakistan.
- Authors
AHMAD, Shoaib; ATIF, Farhan Ahmad; ZAMAN, Arfan; ABBAS, Syed Nazar; KHAN, Yasir Razzaq; RABBANI, Ameer Hamza; SHAHID, Muhammad; NASEER, Omar; ALI, Ahmad; HUSSAIN, Kashif
- Abstract
The study was a prospective trial, planned to determine the molecular epidemiology of ehrlichiosis in dogs through blood smear microscopy and Polymerase chain reaction (PCR). A total of 384 cephalic blood samples were collected from domestic (n=288) and stray dogs (n=96) belonging to varied demographics. Molecular detection of Ehrlichia spp. was conducted through PCR by targeting 16S rRNA gene using the genus specific primers. Final logistic regression analysis revealed that previous history of tick infestation and housing hygiene were significant (P<0.001) risk factors associated with molecular prevalence of canine Ehrlichiosis. The animals of Group-1 (n=7) received only Minocycline at the rate of 12 mg per kg PO 21 days. The dogs of Group-2 (n=7) received Minocycline at the rate of 12 mg per kg PO with Imidocarb dipropionate at the dose rate of 6.6 mg per kg once. Group-3 (n=7) received Ciprofloxacin 10 mg per kg PO only. Group 4 (n=7) received Ciprofloxacin 10 mg per kg along with Prednisolone 1 mg for 8 days. Success of treatment was evaluated based on PCR tests. The results of the treatment trials revealed 71.42%, 85.71%, 57.14% and 71.42 % recovery rate for Group 1, Group 2, Group 3 and Group 4, respectively. The treatment trial concluded that minocycline along with Imidocarb dipropionate produced highest recovery rates.
- Subjects
PAKISTAN; EHRLICHIOSIS; FERAL dogs; DOGS; POLYMERASE chain reaction; DIAGNOSTIC use of polymerase chain reaction; TICK infestations; MOLECULAR phylogeny
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2022, Vol 28, Issue 5, p561
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2022.27559