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- Title
Chemical discovery and global gene expression analysis in zebrafish.
- Authors
Pichler, Franz B; Laurenson, Sophie; Williams, Liam C; Dodd, Andrew; Copp, Brent R; Love, Donald R
- Abstract
The zebrafish (Danio rerio) provides an excellent model for studying vertebrate development and human disease because of itsex utero, optically transparent embryogenesis and amenability toin vivo manipulation. The rapid embryonic developmental cycle, large clutch sizes and ease of maintenance at large numbers also add to the appeal of this species. Considerable genomic data has recently become publicly available that is aiding the construction of zebrafish microarrays, thus permitting global gene expression analysis. The zebrafish is also suitable for chemical genomics, in part as a result of the permeability of its embryos to small molecules and consequent avoidance of external confounding maternal effects. Finally, there is increasing characterization and analysis of zebrafish models of human disease. Thus, the zebrafish offers a high-quality, high-throughput bioassay tool for determining the biological effect of small molecules as well as for dissecting biological pathways.
- Subjects
VERTEBRATE physiology; SOMATIC embryogenesis; GENE expression; ZEBRA danio
- Publication
Nature Biotechnology, 2003, Vol 21, Issue 8, p879
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt852