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- Title
Essential oils of <italic>Citrus aurantifolia</italic>, <italic>Anthemis nobile</italic> and <italic>Lavandula officinalis</italic>: in vitro anthelmintic activities against <italic>Haemonchus contortus</italic>.
- Authors
Ferreira, Luis Eduardo; Benincasa, Bruno Iglesias; Fachin, Ana Lúcia; Contini, Silvia Helena Taleb; França, Suzelei Castro; Chagas, Ana Carolina Souza; Beleboni, Rene Oliveira
- Abstract
Background: Infections of sheep with gastrointestinal parasites, especially <italic>Haemonchus contortus</italic>, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical origin, has grown substantially due to the perspective of less contamination of meat and milk, as well as other advantages related to their cost and accessibility in less developed countries. The aim of this study was to evaluate the in vitro anthelmintic activity of essential oils of the plant species <italic>Citrus aurantifolia</italic>, <italic>Anthemis nobile</italic> and <italic>Lavandula officinalis</italic> against the main developmental stages of the parasite <italic>H. contortus</italic>. Results: Plant species were selected based on substantial ethnopharmacological information. Analysis of the composition of each oil by gas chromatography coupled to mass spectrometry (GC-MS) demonstrated the presence of limonene (56.37%), isobutyl angelate (29.26%) and linalool acetate (35.97%) as the major constituents in <italic>C. aurantifolia</italic>, <italic>A. nobile</italic> and <italic>L. officinalis</italic>, respectively. Different concentrations of each oil were tested in vitro for their capacity to inhibit egg hatching (EHT), larval development (LDT) and adult worm motility (AWMT) using a multidrug-resistant strain of <italic>H. contortus</italic> (Embrapa 2010). The IC50 values obtained for the oils of <italic>C. aurantifolia</italic>, <italic>A. nobile</italic> and <italic>L. officinalis</italic> were 0.694, 0.842 and 0.316 mg/ml in the EHT and 0.044, 0.117 and 0.280 mg/ml in the LDT, respectively. The three oils were able to inhibit adult worm motility completely within the first 8–12 h of observation in the AWMT. Conclusions: The present results demonstrate significant anthelmintic activity of the three oils against the different developmental stages of <italic>H. contortus</italic>. Furthermore, this study is of ethnopharmacological importance by validating the anthelmintic activity of the oils studied. Although new experiments are necessary, these data contribute to the development of pharmaceutical-veterinary products for sheep farming by opening up new therapeutic possibilities against gastrointestinal infections caused by <italic>H. contortus</italic>.
- Subjects
HAEMONCHUS contortus; ESSENTIAL oils; MEXICAN lime; PARASITIC diseases; X-ray diffraction
- Publication
Parasites & Vectors, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1756-3305
- Publication type
Article
- DOI
10.1186/s13071-018-2849-x