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- Title
Effect of Long-Term Normobaric Hyperoxia on Oxidative Stress in Mitochondria of the Guinea Pig Brain.
- Authors
Tatarkova, Zuzana; Engler, Ivan; Calkovska, Andrea; Mokra, Daniela; Drgova, Anna; Hodas, Peter; Lehotsky, Jan; Dobrota, Dusan; Kaplan, Peter
- Abstract
Normobaric hyperoxia (NBO) is applied for treatment of various clinical conditions related to hypoxia, but it can potentially also induce generation of reactive oxygen species, causing cellular damage. In this study, we examined the effects of 60 h NBO treatment on lipid and protein oxidative damage and activity of superoxide dismutase (Mn-SOD) in brain mitochondria of guinea pigs. Despite significant stimulation of Mn-SOD expression and activity the NBO treatment resulted in accumulation of markers of oxidative lesions, including lipid peroxidation (conjugated dienes, thiobarbituric acid reactive substances) and protein modification (bityrosines, adducts with lipid peroxidation products, oxidized thiols). When inhaled O was enriched with oxygen cation, O, the Mn-SOD expression and activity were stimulated to similar extend, but lipid peroxidation and protein oxidation were prevented. These results suggest that long-term NBO treatment causes oxidative stress, but enrichment of inhaled oxygen by oxygen cation can protect the brain again adverse effects of hyperoxia.
- Subjects
HYPEROXIA; DRUG efficacy; CEREBRAL anoxia-ischemia; MITOCHONDRIAL pathology; REACTIVE oxygen species; PROTEIN kinases; SUPEROXIDE dismutase; GUINEA pigs as laboratory animals; OXIDATIVE stress; THERAPEUTICS
- Publication
Neurochemical Research, 2011, Vol 36, Issue 8, p1475
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-011-0473-7