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- Title
Regulation of AMPA Receptor Trafficking by O-Glycosylation.
- Authors
Kanno, Takeshi; Yaguchi, Takahiro; Nagata, Tetsu; Mukasa, Takeshi; Nishizaki, Tomoyuki
- Abstract
The present study investigated the role of O-linked β- N-acetylglucosamine ( O-GlcNAc) glycosylation ( O-GlcNAcylation) in AMPA receptor trafficking. Alloxan, an inhibitor of O-GlcNAc transferase, potentiated responses of AMPA receptors composed of the GluR1 subunit expressed in Xenopus oocytes. No potentiating effect of alloxan was obtained with mutant GluR1 (S831A) receptor lacking CaMKII phosphorylation site. Alloxan facilitated basal synaptic transmission to approximately 120% of basal levels and enhanced Schaffer collateral-CA1 long-term potentiation (LTP) in rat hippocampal slices, especially in the late phase of the LTP. Alloxan stimulated translocation of the GluR1 and GluR2 subunit from the cytosol towards the plasma membrane in rat hippocampal slices with the LTP, although it had no effect on subcellular distribution of the NR1 subunit. Taken together, the results of the present study show that alloxan regulates AMPA receptor trafficking by inhibiting O-GlcNAcylation, to modulate hippocampal synaptic transmission and synaptic plasticity.
- Subjects
RAT physiology; HIPPOCAMPUS diseases; GLYCOSYLATION; NEURAL receptors; NEUROPLASTICITY; CELL membranes
- Publication
Neurochemical Research, 2010, Vol 35, Issue 5, p782
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-010-0135-1