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- Title
Melatonin Supplementation in Extender Enhances the Post Thaw Quality of Buffalo Bull Spermatozoa.
- Authors
Asma-ul-Husna; Ansari, Muhammad Sajjad; Rakha, Bushra Allah; Ejaz, Rabea; Ullah, Nemat; Akhter, Shamim
- Abstract
Oxidative damage to sperm during cryopreservation is one of the main causes of decline in fertilizing potential. Present study aimed at evaluating the role of melatonin in extender for its cryoprotective effect on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50x106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motility, plasma membrane integrity, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to control. In conclusion, the addition of melatonin 0.1-0.5 mM to extender improved the post-thaw quality of buffalo bull semen.
- Subjects
MELATONIN; CATTLE spermatozoa; FROZEN semen; CRYOPRESERVATION of cells; OXIDATIVE stress
- Publication
Pakistan Journal of Zoology, 2017, Vol 49, Issue 1, p163
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2017.49.1.163.167