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- Title
Mammaglobin protein localization and gene expression in the salivary glands.
- Authors
Katsumitsu Shimada; Maya Shimizu; Yuki Ohtani; Roy, Rita R.; Satoshi Murakami; Takanaga Ochiai; Hiromasa Hasegawa; Shimada, Katsumitsu; Shimizu, Maya; Ohtani, Yuki; Murakami, Satoshi; Ochiai, Takanaga; Hasegawa, Hiromasa
- Abstract
<bold>Purpose: </bold>This study aims to delve deeper into the hypothesis that normal salivary gland tissue expresses both protein and mRNA of mammaglobin (MGB).<bold>Methods: </bold>Formalin-fixed paraffin-embedded samples of submandibular (10), parotid (5), palatal (5) and labial glands (30) salivary glands were immunohistochemically investigated. The labial samples were used to examine the MGB positive ratio (MGB-PR), and localize MGB by double immunofluorescence staining and quantitative mRNA gene expression. Mann-Whitney U and Kruskal Wallis rank-sum test for group comparison, and Spearman's rank correlation coefficient for correlation analysis were used.<bold>Results: </bold>The distribution of MGB-positive cells was variable throughout samples with significantly higher MGB-PR of acini than ducts (P = 0.00376), and there was no difference when compared based on age (P = 0.0646) and gender (P = 0.245). Besides acinar cells, a number of myoepithelial cells and ductal cells also demonstrated strong MGB reactivity with varying MGB mRNA expression levels in 6 of the 7 samples (with MGB-PR > 20%) tested.<bold>Conclusion: </bold>This novel study shows that unlike aberrant protein expression in some carcinomas, MGB expression in salivary gland neoplasms represents the nature of original cells, giving a better insight into the neoplasms expressing MGB.
- Publication
Journal of Oral Science, 2021, Vol 63, Issue 4, p310
- ISSN
1343-4934
- Publication type
journal article
- DOI
10.2334/josnusd.21-0180