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- Title
Interferon-Mediated Enhancement of Thyroid Major Histocompatibility Complex Antigen Expression.
- Authors
Rayner, D. C.; Lydyard, P. M.; De Assis-Paiva, H. J.; Bidey, S.; van der Meide, P.; Varey, A.-M.; Cooke, A.
- Abstract
Epithelial expression of class II antigens encoded by the major histocompatibility complex (MHC) has been proposed as a means by which autoimmune thyroid disease may be initiated and maintained. We studied a rat thyroid epithelial cell line (FRTL-5), which constitutively expresses class I (OX18) but not class II (OX6 or OX17) determinants to quantify in vitro MHC antigen induction using flow cytometry. Recombinant rat y interferon (rIFN-γ) induced dose- dependent expression of OX6 (I-A) antigen at >48 h (maximum 80–90% of cells in culture at 100 U/ml), which was abrogated by DB-1, a monoclonal antibody to rat IFN-γ, OX17 antigen (I-E) was also induced (86%) and OX18 (class I) markedly increased under these conditions. Other thyroid-aclive agents including the calcium ionophore A23187, dibutyryl cyclic AMP, thyroid-stimulating autoantibodies from Graves' disease patients (LATS), and TSH, caused no I-A induction. Supernatants from spleen cells stimulated with plant lectins (concanavalin A or phytohaemagglutinin), but not lectin alone, evoked substantial class II induction, which was inhibited by DB-1. These findings suggest that IFN-γ is the central mediator of thyroid epithelial class II expression. FRTL-5 provides a powerful model for the analysis of thyroid MHC class II dynamics and a potential means of analysing the role of epithelial class II in autoimmune pathogenesis.
- Subjects
HLA histocompatibility antigens; INTERFERONS; THYROID gland; IMMUNOLOGY; MAJOR histocompatibility complex; IMMUNOGENETICS; ANTINEOPLASTIC agents
- Publication
Scandinavian Journal of Immunology, 1987, Vol 25, Issue 6, p621
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1987.tb01088.x