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- Title
Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine against brucellosis.
- Authors
Rezaei, Marzieh; Rabbani Khorasgani, Mohammad; Zarkesh Esfahani, Sayyed Hamid; Emamzadeh, Rahman; Abtahi, Hamid
- Abstract
The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with appropriate restriction sites and subcloned in pAMJ2008 expression vector digested with the same enzymes. The purified recombinant constructed pAMJ-rOmp-IL2 was introduced into L. lactis subsp. cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2 fusion protein was investigated. This study reports the construction of a recombinant L. lactis expressing the Omp16-IL2 fusion protein as an oral Lactococcus-based vaccine, as compared with commonly used live attenuated vaccines, for future studies against brucellosis.
- Subjects
LACTOCOCCUS lactis; BRUCELLA melitensis; CHIMERIC proteins; ORAL vaccines; VACCINES
- Publication
Canadian Journal of Microbiology, 2020, Vol 66, Issue 1, p39
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2019-0261