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- Title
Purification and Antioxidant Activity of Polysaccharide from Zizyphus jujuba Mill in Xinjiang.
- Authors
WU Na; YANG Jie; XU Hai-yan; SHENG Xiao-li
- Abstract
This pape studied on the purification and antioxidant properties of polysaccharide from Zizyphus jujuba Mill. Crude polysaccharide was obtained from Zizyphus jujuba Mill using following methods: extracting polysaccharide with hot water, precipitating with ethyl alcohol. Free protein and pigment were removed by Sevage method and H2O2. Three polysaccharide fractions were gotten by DEAE-Cellulose column with eluent of water, 0-1 mol/L NaCl. JPSN, JPSA1 ,JPSA2 was further purified on Sephadex G-100 column, three subfractions of JPSA2 (JPSA2 B1, JPSAzB2t, JPSA2B3) were obtained. And JPSN, JPSA would be studied too. Sephadex G-100 column chromatography and pc illustrated that the fraction JPSN, JPSA1 was homogenous polysaccharide. The results demonstrated that polysaccharides have antioxidant properties which may scavenge DPPH, superoxide radicals, hydroxyl radical.
- Subjects
XINJIANG Uygur Zizhiqu (China); CHINA; POLYSACCHARIDES; ZIZIPHUS; CHEMICAL purification; ANTIOXIDANTS
- Publication
Natural Product Research & Development, 2009, Vol 21, Issue 2, p319
- ISSN
1001-6880
- Publication type
Article