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- Title
VEGF Cancer Biomarker Protein Detection in Real Human Serum Using Capacitive Label-Free Aptasensor.
- Authors
Qureshi, Anjum; Roci, Irena; Gurbuz, Yasar; Niazi, Javed H.
- Abstract
Vascular Endothelial Growth Factor (VEGF) is a signalling protein known as a serum biomarker for a number of diseases including cancer. Therefore, it is necessary to develop a label-free, rapid and sensitive method for detecting VEGF in its native forms in human serum. In this work, we have demonstrated the proof-of-concept of a sensitive label-free biosensor based on capacitance changes induced by capturing of VEGF in human serum using anti-VEGF aptamers functionalized on interdigitated (IDE) capacitor arrays (aptasensors). Limit of detection of VEGF protein using this aptasensor was in the range 0.5-2 ng/mL in dilute human serum. The label-free capacitive aptasensing strategy provides a simple, reliable and effective means of biosensing serum VEGF levels for early cancer diagnosis.
- Subjects
VASCULAR endothelial growth factors; SERUM; BIOSENSOR research; CANCER diagnosis; PROOF of concept
- Publication
Macromolecular Symposia, 2015, Vol 357, Issue 1, p74
- ISSN
1022-1360
- Publication type
Article
- DOI
10.1002/masy.201400191