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- Title
A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.
- Authors
Gadkar, Vijay; Driver, James D.; Rillig, Matthias C.
- Abstract
The Petri plate dual in vitro culture system for arbuscular mycorrhizal fungi (AMF) is ill-suited for the production and isolation of extraradically released soluble factors, thereby preventing the further characterization of such compounds. To overcome this technical bottleneck, we here describe a novel cultivation system using the standard AMF Glomus intraradices-carrot dual culture as a model. This new system is capable of producing soluble factors in copious amounts without any interference from host roots. The greatest advantages of this culture method include ease of handling and reusability of the culture vessel, thus making it a very cost effective system.
- Subjects
MYCORRHIZAL fungi; HYPHAE of fungi; SOIL fungi; ENDOGONALES; CRYPTOGAMS; MYCELIUM
- Publication
Biotechnology Letters, 2006, Vol 28, Issue 14, p1071
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-006-9055-1