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- Title
Reconditioned monocytes are immunomodulatory and regulate inflammatory environment in sepsis.
- Authors
Jain, Kshama; Mohan, K. Varsha; Roy, Gargi; Sinha, Prakriti; Jayaraman, Vignesh; Kiran; Yadav, Ajit Singh; Phasalkar, Akshay; Deepanshu; Pokhrel, Anupa; Perumal, Nagarajan; Sinha, Nitin; Chaudhary, Kiran; Upadhyay, Pramod
- Abstract
Sepsis is caused by dysregulated immune response to severe infection and hyper inflammation plays a central role in worsening the disease. The immunomodulatory properties of mesenchymal stem cells (MSCs) have been evaluated as a therapeutic candidate for sepsis. Reconditioned monocytes (RM), generated from healthy human peripheral blood mononuclear cells (PBMCs) exhibit both macrophage and MSCs-like properties. RM were administered at different stages of sepsis in a mouse model. It reduced serum levels of IL6, MCP-1, IL-10, improved hypothermia, increased survival, and recovery from 0 to 66% when combined with antibiotics in the mouse model. The reduced human leucocyte antigen DR molecules expression on RM enables their co-culture with PBMCs of sepsis patients which resulted in reduced ROS production, and up-regulated TGF-β while down-regulating IL6, IL8, and IL-10 in-vitro. RM are potentially immunomodulatory, enhance survival in sepsis mouse model and modulate inflammatory behaviour of sepsis patient's PBMCs.
- Subjects
MONONUCLEAR leukocytes; SEPSIS; MONOCYTES; LEUCOCYTES; MESENCHYMAL stem cells
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-42237-4