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- Title
Simian virus 40-mediated cis induction of the Xenopus β-globin DNase I hypersensitive site.
- Authors
Enver, Tariq; Brewer, Alison C.; Patient, Roger K.
- Abstract
Regions in chromatin which are hypersensitive to the action of DNase I appear to be associated with sites of genetic activity; the association between DNase I hypersensitivity and transcriptional activation is well known1. In the case of the chicken β-globin gene the establishment of a DNase I hypersensitive site is dependent on tissue-specific trans-acting factors2,3. Such factors have also been implicated in the action of viral and cellular enhancers4-10, which are themselves hypersensitive to DNase I11-14. Enhancers have been defined operationally as DNA sequences which act in cis to potentiate transcription from their own, heterologous or cryptic promoters. This activity is essentially unaffected by changes in the orientation, position (5′ or 3′) or distance of the enhancer element with respect to its cognate promoter (ref. 15 for review). We demonstrate here that the transcriptional rescue of the Xenopus laevis β-globin gene by simian virus 40 (SV40) sequences including the enhancer coincides with the conferment of DNase I hypersensitivity upon that gene, and that this occurs in the absence of any change in the complement of trans-acting factors. These results suggest that a propensity to form sites hypersensitive to the action of DNase I is encoded in the primary sequence of DNA16, and that this predilection is aggravated by SV40 sequences, perhaps through a mechanism dependent on supercoiling.
- Publication
Nature, 1985, Vol 318, Issue 6047, p680
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/318680a0