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- Title
Frequency of Toxoplasma gondii in the retina in eye banks in Brazil.
- Authors
Costa, Deise; Nascimento, Heloisa; Sutili, Aline; Nobrega, Fernando; Fowler, Flavio; Nobrega, Mario; Garrido, Cristina; Oliveira Dias, Janaina; Adán, Consuelo; Rizzo, Luiz; Silveira, Claudio; Belfort, Rubens; Commodaro, Alessandra
- Abstract
Ocular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus ( n = 60), Sao Paulo ( n = 60), Chapeco ( n = 26), and Joinville ( n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).
- Subjects
EYE banks; TOXOPLASMA gondii; UVEITIS; NUCLEIC acid isolation methods; CHOROIDITIS
- Publication
Parasitology Research, 2017, Vol 116, Issue 7, p2031
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-017-5474-2