We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Optimization of Cultivation Conditions of Yarrowia lipolytica Po1f pUV3-Op Transformant to Get the Encapsulated Phytase as The Feed Supplement.
- Authors
Gessler, N. N.; Kokoreva, A. S.; Deryabina, Yu. I.; Isakova, E. P.
- Abstract
The study presents the results of optimizing the medium components for the cultivation of the recombinant yeast of Yarrowia lipolytica Po1f pUV3-Op to increase the biomass concentration and its phytase activity. Glycerol, yeast extract, and the broth from low-value raw waste of the poultry farms were used as the substrates in the nutrient medium. During the cultivation on the developed nutrient medium in a 10-litre laboratory fermenter under the pilot conditions the optimal fermentation parameters were tested. The yield biomass under the optimized conditions reached 292 g of dried preparation with a phytase activity of 100.1 ± 1.07 mmol Pi × min–1 × g–1 of dry biomass.
- Subjects
DIETARY supplements; PHYTASES; POULTRY farms; YEAST extract; AGRICULTURAL wastes; BIOMASS
- Publication
Applied Biochemistry & Microbiology, 2023, Vol 59, Issue 6, p909
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683823330013