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- Title
Coordination Ion Spray for Analysis of the Growth Hormones Releasing Peptides in Urine—An Application Study.
- Authors
Temerdashev, Azamat; Gashimova, Elina; Azaryan, Alice; Feng, Yu-Qi; Atapattu, Sanka N.
- Abstract
In this article, a comparison of ionization techniques is provided and discussed. Conventional liquid chromatography with an electrospray ionization source shows higher robustness and repeatability in comparison with liquid chromatography coupled with a coordination ion spray (CIS-MS) source using silver nitrate as the dopant. However, the higher sensitivity and possibility to collect more data in untargeted applications mean CIS-MS is emerging as an instrument used in specific applications. During this research, the limit of detection (LOD) for GHRP-2 and GHRP-6 was established at 0.2 ng/mL, and the lower limit of quantification (LLOQ) was 0.5 ng/mL for CIS-MS. For conventional ESI-MS combined with solid-phase extraction on weak cation exchange columns, the limit of detection was found to be 1 ng/mL, and the lower limit of quantification was 2 ng/mL.
- Subjects
ION analysis; SOMATOTROPIN; PEPTIDES; SOLID phase extraction; LIQUID chromatography
- Publication
Separations (2297-8739), 2024, Vol 11, Issue 5, p155
- ISSN
2297-8739
- Publication type
Article
- DOI
10.3390/separations11050155