We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Purification and Characterization of a Lectin from Bulbs of Cyclamen Mirabile.
- Authors
Kocazorbaz, Ebru Kocadağ; Azbazdar, Ayşe Yağmur; Öztürk, Merve Aliakar; Menfaatli, Esra
- Abstract
Lectin from the bulbs of Cyclamen mirabile which is an endemic species of Turkey was successfully isolated by affinity precipitation with alginate in one step. The purified protein produced two bands showing a dimeric structure in SDS-PAGE (13.5 and 14.8 kDa). C. mirabile lectin showed activity and stability in a broad pH scale and kept its haemagglutination activity in the temperature range of 4-40°C. MgCl2 and HgCl2 inhibited the haemagglutination activity of the lectin. In this study, a practical and efficient purification procedure was carried out for C. mirabile lectin by using affinity precipitation with alginate.
- Subjects
TURKEY; CYCLAMEN; LECTINS; SODIUM dodecyl sulfate; HYDROGEN-ion concentration
- Publication
Bilecik Seyh Edebali University Journal of Science / Bilecik Şeyh Edebali Üniversitesi Sosyal Bilimler Dergisi, 2021, Vol 8, Issue 1, p1
- ISSN
2458-7575
- Publication type
Article
- DOI
10.35193/bseufbd.685652