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- Title
下调 FoxM1 基因表达对肾癌 786-O 细胞生物学行为的影响.
- Authors
程鑫宇; 吴小荣; 贾博深; 沙建军; 陈勇辉; 黄吉炜; 张进; 刘东明; 黄翼然
- Abstract
Objective To observe the down-regulation of forkhead box protein M1 (FoxM1) gene expression and to investigate its effect on the biological behavior of human renal cancer cell line 786-O. Methods The shFoxM1 plasmid was constructed by RNA interference sequence and shRNA .786-O cells were divided into the shFoxM1 group and the control group which were transfected with shFoxM1 plasmid and scramble plasmid for 48 h, respectively. The expression of FoxM1 mRNA and protein was tested by real-time PCR and Western blotting. Then we analyzed the efficiency of plate colony formation in one week by using colony formation assay, and we used flow cytometry to detect the cell cycle and apoptosis of 786-O cells. The migration and invasion abilities were analyzed by Transwell assay, and the OD value in 0, 24, 48 and 72 hours was tested using MTT assay to analyze the activity of proliferation. Results Compared with the control group, the FoxM1 mRNA and protein expression was decreased, the efficiency of plate colony formation and the cell proliferation were decreased, the cells were blocked in G1 phase, cells of G2 phase and S phase reduced, the early apoptosis rate rose and the migration and invasion cells were decreased in the shFoxM1 group (all P<0.05). Conclusions Down-regulating FoxM1 gene expression suppressed the proliferation , migration and invasion abilities of 786-O cells, blocking cells in G1 phase and promoting early apoptosis.
- Publication
Shandong Medical Journal, 2015, Vol 55, Issue 30, p1
- ISSN
1002-266X
- Publication type
Article
- DOI
10.3969/j.issn.1002-266X.2015.30.001