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- Title
高温和高盐对Nitrosomonas nitrosa WH-1氨氧化活性的联合影响.
- Authors
程凯; 杨睿
- Abstract
Autotrophic ammonia-oxidizing bacteria are important bacteria in wastewater treatment, but studies on the combined effects of high temperature and high salt on their ammonia-oxidizing activity are still relatively scarce. In this paper, with a purified ammonia oxidizing bacteria Nitrosomonas nitrosa WH-1 as the research object, the combined effects of temperature and salt concentration on nitrite accumulation rate were determined by response surface methodology. According to the nitrite accumulation rate under different salt concentrations (3~18 g·L-1) and different temperatures (36 ℃~48 ℃) conditions, optimum temperature, IC50 of temperature and IC50 of salt concentration to the ammoxidation activity was calculated. The results indicated that there was an significant interaction between high temperature and high salinity on the NO2--N accumulate rate. When the temperature increased from 36 ℃ to 42 ℃, the IC50 of salt concentration increased from 8.84 g·L-1 to 10.45 g·L-1. Moreover, the optimum temperature and the IC50 of high temperature were significantly higher when the salt concentration was 15~18 g·L-1 comparing to the salt concentration of 3~12 g·L-1. The above results suggested that high temperature would alleviate the inhibition caused by high salinity, and moderate high salinity would also improve the heat resistance of ammonia oxidizing bacteria.
- Subjects
AMMONIA-oxidizing bacteria; AUTOTROPHIC bacteria; RESPONSE surfaces (Statistics); HIGH temperatures; TEMPERATURE effect; SALT-free diet
- Publication
Journal of Central China Normal University, 2023, Vol 57, Issue 1, p152
- ISSN
1000-1190
- Publication type
Article
- DOI
10.19603/j.cnki.1000-1190.2023.01.015