We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Kainic Acid and MPP<sup>+</sup> Induce Upregulation of GLT-1 in Neuroblastoma and Glia Cells.
- Authors
UYARICI, Rabia; AK, Ceylan; DÖNMEZ YALÇIN, Gizem
- Abstract
Objective: Glutamate is the major excitatory transmitter in the brain. The excessive glutamate will lead to excitotoxicity. Glutamate transporter-1 (GLT-1) is the major transporter that performs 95% of the glutamate clearance contributing to normal neuronal function and preventing excitotoxicity. In this study, we investigated the effect of two toxins, kainic acid and MPP+ (1-methyl-4-phenylpyridinium), on GLT-1 expression and excitotoxicity in neuroblastoma and glia cells (immortalized human astrocytes). Methods: We treated neuroblastoma and glia cells with kainic acid and MPP+, applied MTT assay to measure the cell viability. We identified the mRNA and protein levels of GLT-1 and also analyzed released glutamate levels using glutamate assay. Results: The mRNA level of GLT-1 increased in neuroblastoma cells as a result of kainic acid or MPP+ treatment while the protein expression of GLT-1 increased in glia cells after the treatment with MPP+. Excess glutamate was found to be decreased after 12 h MPP+ treatment. However, this decrease was no more prominent with further MPP+ treatment. Conclusion: Our results show that GLT-1 levels are elevated as a result of kainic acid or MPP+ treatment as a survival mechanism to prevent excitotoxicity.
- Subjects
KAINIC acid; NEUROBLASTOMA; PROTEIN expression; GLUTAMIC acid; CELL survival; BACTERIAL toxins
- Publication
Bezmialem Science, 2021, Vol 9, Issue 2, p164
- ISSN
2148-2373
- Publication type
Article
- DOI
10.14235/bas.galenos.2020.4032