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- Title
Transcriptome Analysis of Propylaea quatuordecimpunctata L. (Coleoptera: Coccinellidae) under High Temperature Stress.
- Authors
Yang, Qing; Liu, Jinping; Yang, Yizhong; Lu, Yanhui
- Abstract
Simple Summary: Propylaea quatuordecimpunctata (L.), an important natural enemy of crop pests, shows different levels of survival, reproduction, and predation at different high temperatures (32, 35, and 38 °C). Therefore, it is important to understand the molecular basis for this variation in the specie's thermotolerance. In this study, high-throughput sequencing analysis confirmed that P. quatuordecimpunctata has more genes which are mobilized under high temperature (38 °C). Meanwhile, we found that the cytochrome P450 gene and the heat shock protein Hsp70 gene were significantly overexpressed after high temperature stress (35 and 38 °C). The reliability of transcriptome sequencing was confirmed by qRT-PCR, indicating that these differentially expressed genes (DEGs) play an important role in the resistance of P. quatuordecimpunctata to high temperature stress. Our study provides a molecular basis for further analysis of the thermotolerance mechanism of P. quatuordecimpunctata. In cotton-growing regions of northern Xinjiang, Propylaea quatuordecimpunctata (L.) (Coleoptera: Coccinellidae) is an important natural enemy that provides significant control of some pest hemipterans. Previous studies have shown that the survival and reproduction of P. quatuordecimpunctata differs under different high temperatures. However, its molecular mechanism for thermotolerance is poorly understood. In this study, transcriptome sequencing analysis was performed on P. quatuordecimpunctata, after its exposure to different temperatures (32–38 °C) for 24 h, using high-throughput sequencing technology. The results showed that the 35 vs. 38 °C groups had the most DEGs (1425), indicating that P. quatuordecimpunctata has more genes that can be mobilized under high temperature (38 °C). The results of functional analysis showed that the DEGs were mainly involved in "Oxidation–reduction process", "Oxidoreductase activity", "Metabolic pathways", and "Small molecule metabolic processing" groups. We randomly selected DEGs (eleven P450 genes and one Hsp70 gene) of interest for qRT-PCR validation. The qRT-PCR results were consistent with the transcriptome data, indicating that the transcriptome data were reliable. In summary, these genes involved in these pathways play an important role in the resistance of P. quatuordecimpunctata to high temperature stress. Our study enriched our understanding of the molecular mechanism for thermotolerance in P. quatuordecimpunctata.
- Subjects
XINJIANG Uygur Zizhiqu (China); HSP70 heat-shock proteins; HIGH temperatures; LADYBUGS; TRANSCRIPTOMES; SMALL molecules
- Publication
Agriculture; Basel, 2022, Vol 12, Issue 8, pN.PAG
- ISSN
2077-0472
- Publication type
Article
- DOI
10.3390/agriculture12081088