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- Title
LACTIC ACID PRODUCTION FROM DRIED DISTILLER'S GRAINS WITH SOLUBLES HYDROLYSATE VIA CO-FERMENTATION OF Lactobacillus pentosus AND Lactobacillus coryniformis.
- Authors
ZAINI, N. A. M.; CHARALAMPOPOULOS, D.; CHATZIFRAGKOU, A.
- Abstract
Dried Distillers Grains with Soluble (DDGS) is a by-product of bioethanol and distillery industries that is currently marketed as animal feed. DDGS are characterised by high protein and fibre (lignocellulose) content, which shows potential to be utilised as alternative feedstock for microbial fermentations. Lignocellulose hydrolysate is commonly composed of a mixture of hexose and pentose sugars, where the former is always preferable by most of microorganisms but not favourable in economical perspectives. Therefore, the main objective of this study was to optimise the utilisation of DDGS hydrolysate by incorporating pentose-fermenting microorganism together with hexose-fermenting microorganism (Lactobacillus coryniformis) during lactic acid fermentation. Two pentose-fermenting strains, Lactobacillus pentosus and Lactobacillus brevis were analysed for their lactic acid production in MRS broth (to determine the optimal bacterial growth conditions) and DDGS hydrolysate. Result shows that both strains grew well in MRS media, but only L. pentosus can utilise DDGS hydrolysate with 24.9 g/L lactic acid was produced after 18 hr fermentation. In co-fermentation experiment, simultaneous and sequential cultivation technique of L. pentosus with L. coryniformis were tested. Simultaneous co-fermentation of both strains in DDGS hydrolysate exhibited higher lactic acid production with 28.5 g/L lactic acid (18 hr) that corresponded to 83.3% yield. In conclusion, efficient utilisation of both cellulose and hemicellulose components from DDGS hydrolysate employing mixed culture fermentation improved overall lactic acid production.
- Subjects
LACTIC acid bacteria; LACTOBACILLUS; PROBIOTICS
- Publication
Malaysian Applied Biology, 2018, Vol 47, Issue 5, p173
- ISSN
0126-8643
- Publication type
Article