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- Title
Suitability of macrophage inflammatory protein-1β production by THP-1 cells in differentiating skin sensitizers from irritant chemicals.
- Authors
Yeon-Mi Lim; Seong-Joon Moon; Su-Sun An; Soo-Jin Lee; Seo-Young Kim; Ih-Seop Chang; Kui-Lea Park; Hyoung-Ah Kim; Yong Heo
- Abstract
Background: Worldwide restrictions in animal use for research have driven efforts to develop alternative methods. Objective: The study aimed to test the efficacy of the macrophage inflammatory protein-1β (MIP-1β) assay for testing chemicals’ skin-sensitizing capacity. Methods: The assay was performed using 9 chemicals judged to be sensitizing and 7 non-sensitizing by the standard in vivo assays. THP-1 cells were cultured in the presence or absence of 4 doses, 0.01x, 0.1x, 0.5x, or 1x IC50 (50% inhibitory concentration for THP-1 cell proliferation) of these chemicals for 24 hr, and the MIP-1β level in the supernatants was determined. Skin sensitization by the test chemicals was determined by MIP-1β production rates. The MIP-1β production rate was expressed as the relative increase in MIP-1β production in response to chemical treatment compared with vehicle treatment. Results and Conclusion: When the threshold MIP-1β production rate used was 100% or 105% of dimethyl sulfoxide, all the sensitizing chemicals tested (dinitrochlorobenzene, hexyl cinnamic aldehyde, eugenol, hydroquinone, dinitrofluorobenzene, benzocaine, nickel, chromium, and 5-chloro-2-methyl-4-isothiazolin-3-one) were positive, and all the non-sensitizing chemicals (methyl salicylate, benzalkonium chloride, lactic acid, isopropanol, and salicylic acid), with the exception of sodium lauryl sulfate, were negative for MIP-1β production. These results indicate that MIP-1β could be a biomarker for classification of chemicals as sensitizers or non-sensitizers.
- Subjects
CHEMICAL terrorism; CHEMICALS; INORGANIC chemistry; CELL proliferation; CELL growth; ORGANIC compounds
- Publication
Contact Dermatitis (01051873), 2008, Vol 58, Issue 4, p193
- ISSN
0105-1873
- Publication type
Article
- DOI
10.1111/j.1600-0536.2007.01311.x