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- Title
High-Throughput Automated Microscopy of Circulating Tumor Cells.
- Authors
Aguilar-Avelar, Carlos; Soto-García, Brenda; Aráiz-Hernández, Diana; Yee-de León, Juan F.; Esparza, Miguel; Chacón, Franco; Delgado-Balderas, Jesús Rolando; Alvarez, Mario M.; Trujillo-de Santiago, Grissel; Gómez-Guerra, Lauro S.; Velarde-Calvillo, Liza P.; Abarca-Blanco, Alejandro; Wong-Campos, J. D.
- Abstract
Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures. An excellent agreement between our machine vision-based approach and a state-of-the-art microscopy equipment was achieved. Our integral approach provides a path for operator-free and robust analysis of cancer cells as a standard clinical practice.
- Subjects
CANCER diagnosis; FLUORESCENCE microscopy; HETEROGENEITY; CANCER cells; IMMUNOSTAINING; FLUOROPHORES
- Publication
Scientific Reports, 2019, Vol 9, Issue 1, pN.PAG
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-019-50241-w