We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination.
- Authors
Lee, Do-Wan; Kwon, Jae-Im; Woo, Chul-Woong; Heo, Hwon; Kim, Kyung Won; Woo, Dong-Cheol; Kim, Jeong Kon; Lee, Dong-Hoon
- Abstract
This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (p = 0.001), gamma-aminobutyric acid (p = 0.019), and glutamate + glutamine (p = 0.001); however, creatine + phosphocreatine (p = 0.006) and myo-inositol (p = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (p < 0.001), glutamate/total creatine (p < 0.001), and GABA/total creatine (p = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.
- Subjects
PROTON magnetic resonance spectroscopy; SPRAGUE Dawley rats; DEMYELINATION; HIPPOCAMPUS (Brain); GABA; MYELIN sheath diseases
- Publication
Diagnostics (2075-4418), 2021, Vol 11, Issue 1, p45
- ISSN
2075-4418
- Publication type
Article
- DOI
10.3390/diagnostics11010045