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- Title
Hepatocyte growth factor gene transfer into the liver via the portal vein using electroporation attenuates rat liver cirrhosis.
- Authors
Matsuno, Y; Iwata, H; Umeda, Y; Takagi, H; Mori, Y; Kosugi, A; Matsumoto, K; Nakamura, T; Hirose, H
- Abstract
Although a variety of gene transfer methods to the liver have been designed, there are some problems such as the transfection efficiency and safety. In the present study, we developed a modified method of gene transfer into the liver by infusion of plasmid DNA via the portal vein followed by electroporation. After green fluorescence protein gene transfer, transgene expressions were detected in 24 h, and then maximally at 3 days, and persisted for 3 weeks. Histological analysis revealed that very mild tissue damage was induced in the liver to which electroporation was applied. In the second study, human hepatocyte growth factor (HGF) was more detected in the liver injected with 500 µg of human HGF gene than 100 µg of human HGF gene. However, serum HGF did not increase with 100 or 500 µg of human HGF gene. Moreover, 500 µg of HGF gene transfer into the liver by using this method could achieve the long survival of all dimethylnitrosamine-treated rats and attenuate the fibrous regions in the liver. These results suggest that HGF gene transfer into the liver via the portal vein using electroporation might be one of the useful methods for the treatment of various liver diseases.
- Subjects
GENETIC transformation; ELECTROPORATION; HEPATOCYTE growth factor; LIVER diseases; PORTAL vein diseases
- Publication
Gene Therapy, 2003, Vol 10, Issue 18, p1559
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3302052