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- Title
Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila.
- Authors
Thanh Dien, Le; Linh, Nguyen Vu; Sangpo, Pattiya; Senapin, Saengchan; St‐Hilaire, Sophie; Rodkhum, Channarong; Dong, Ha Thanh
- Abstract
A rapid increase in multi‐drug‐resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB‐O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB‐O3 exposures caused alterations to the gills of exposed fish. Here, we set up a modified recirculation system (MRS) assembled with an NB‐O3 device (MRS‐NB‐O3) to investigate whether MRS‐NB‐O3 (a) were safe for tilapia (Oreochromis niloticus), (b) were effective at reducing bacterial load in rearing water and (c) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results showed no behavioural abnormalities or mortality of Nile tilapia during the 14‐day study using the MRS‐NB‐O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/ml) was used, multiple NB‐O3 treatments in the first two days reduced the bacteria between 15.9% and 35.6% of bacterial load in water, while bacterial concentration increased from 13.1% to 27.9% in the untreated control. There was slight up‐regulation of non‐specific immune‐related genes in the gills of the fish receiving NB‐O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percentage survival (RPS) of 64.7% – 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB‐O3 is a promising non‐antibiotic approach to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).
- Subjects
NILE tilapia; AEROMONAS hydrophila; OZONE; PUBLIC health; PATHOGENIC bacteria; BACTERIAL diseases; TILAPIA
- Publication
Journal of Fish Diseases, 2021, Vol 44, Issue 9, p1435
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/jfd.13451