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- Title
miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus.
- Authors
Feng, Meng-Qi; Lu, Meng-Di; Long, Jian-Mei; Yin, Zhao-Ping; Jiang, Nan; Wang, Peng-Bo; Liu, Yue; Guo, Wen-Wu; Wu, Xiao-Meng
- Abstract
Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156- SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156- SPL module in SE remains unclear. In this study, we found that transcription factors CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (CsSPL) genes, and reduction of SE efficiency. In the short tandem target mimic (STTM)-miR156a overexpression callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated. csi-miR172d was down-regulated, whereas the target genes, CsTOE1.1 and CsTOE1.2 , which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs , thus altering starch accumulation in callus cells and regulating SE in citrus. This study elucidates the pathway of miR156- SPLs and miR172- TOEs -mediated regulation of SE, and provides new insights into enhancing SE capability in citrus.
- Subjects
STARCH; CITRUS; PLANT biotechnology; AMYLOPLASTS; PLANT cells &; tissues; SOMATIC embryogenesis
- Publication
Journal of Experimental Botany, 2022, Vol 73, Issue 18, p6170
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erac248