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- Title
The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice.
- Authors
Yi Chen; Sheng-Kai Sun; Zhong Tang; Guidong Liu; Moore, Katie L.; Maathuis, Frans J. M.; Miller, Antony J.; McGrath, Steve P.; Fang-Jie Zhao
- Abstract
Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 pM As(III), but enhanced by 20 and 100 pM As(III). Transgenic rice plants expressing OsNIP3;2pro-GUS showed that the gene was predominantly expressed in the lateral roots and the stele region of the primary roots. Transient expression of OsNIP3;2:GFP fusion protein in rice protoplasts showed that the protein was localized in the plasma membrane. Knockout of OsNIP3;2 significantly decreased As concentration in the roots, but had little effect on shoot As concentration. Synchrotron microfocus X-ray fluorescence showed decreased As accumulation in the stele of the lateral roots in the mutants compared with wild-type. Our results indicate that OsNIP3;2 is involved in As(III) uptake by lateral roots, but its contribution to As accumulation in the shoots is limited.
- Subjects
NODULINS; MEMBRANE proteins; ARSENITES; RICE; X-ray fluorescence
- Publication
Journal of Experimental Botany, 2017, Vol 68, Issue 11, p3007
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erx165