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- Title
Phytochemical Analysis and Central Effects of Annona Muricata Linnaeus: Possible Involvement of the Gabaergic and Monoaminergic Systems.
- Authors
Souza, Daniele Oliveira; dos Santos Sales, Valterlúcio; de Souza Rodrigues, Cristina Kelly; de Oliveira, Larissa Rolim; Lemos, Izabel Cristina Santiago; de Araújo Delmondes, Gyllyandeson; Monteiro, Álefe Brito; do Nascimento, Emmily Petícia; Nóbrega de Figuêiredo, Francisco Rodolpho Sobreira Dantas; da Costa, José Galberto Martins; da Cruz, Giovany Michely Pinto; de Barros Viana, Glauce Socorro; Barbosa, Roseli; de Menezes, Irwin Rose Alencar; Felipe, Cícero Francisco Bezerra; Kerntopf, Marta Regina
- Abstract
Annona muricata Linnaeus (Annonaceae), popularly known as graviola, is used in folk medicine as both sedative and anticonvulsant. This study correlates the neurochemical profile with the behavioral effects of the hydroalcoholic extract from the leaves of Annona muricata (HLEAM) in mice, proposing to elucidate their mechanism of action on the central nervous system. Flavonoids and phenolic compounds were identified and quantified by High Performance Liquid Chromatography (HPLC) method. The acute toxicity (median lethal dose - LD50) was determined by probitos method using the percentage of mortality based on the Hippocratic screen. HLEAM (25, 50 and 100 mg/kg) was tested, intraperitoneally (i.p.), in models of sedation, anxiety, motor coordination, and seizures. The endogenous levels of dopamine, norepinephrine and DOPAC were assayed by reverse-phase HPLC with electrochemical detection. The HPLC analysis of the extract revealed the presence of flavonoids (quercetin, isoquercitrin, quercitrin, rutin, and kaempferol) and phenolics acids (gallic, chlorogenic, ellagic and caffeic acids). The LD50 was 1091.7 mg/kg and Hippocratic screening indicated central nervous system depressant effect. HLEAM presented sedative effects at doses of 25, 50, and 100 mg/kg, as well as anxiolytic and anticonvulsant effects at a dose of 100 mg/kg. In addition, these effects were partially reversed by flumazenil. The monoamines analysis by HPLC showed that HLEAM decreased the level of norepinefrine and dopamine in the mouse brain striatum. Thus, the results indicate a possible interaction of HLEAM with the GABAergic and monoaminergic systems, adding medicinal value to the popular use of the plant for the treatment of behavioral and neurological disorders.
- Subjects
ANNONACEAE; PHYTOCHEMICALS; GABA agents; HIGH performance liquid chromatography; CAFFEIC acid
- Publication
Iranian Journal of Pharmaceutical Research, 2018, Vol 17, Issue 4, p1306
- ISSN
1735-0328
- Publication type
Article