We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs.
- Authors
Xi Feng; Yanan Li; Chang Tian; Wei Yang; Xinyu Liu; Changyi Zhang; Zhirui Zeng
- Abstract
Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae, we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae, namely, Saccharolobus, Sulfolobus, and Metallosphaera. We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies.
- Subjects
HOT springs; VIRUS isolation; MEMBRANE lipids; VIRAL envelopes; VIRUS-like particles; ETHER lipids; VIRAL envelope proteins
- Publication
Frontiers in Microbiology, 2023, Vol 14, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2023.1134935