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- Title
Gold Nanoparticles as Dual Functional Sensor to Detect E.coliDH5α as a Model for Gram-negative Bacteria.
- Authors
Ma, Qun; Gong, Nianchun; Li, Yanle; Jiang, Xi; Yin, Li; Chen, Xueyin; Huan, Shuangyan
- Abstract
4-aminothiophenol-modified gold nanoparticles (PATP-AuNPs) were used as colorimetric and Surface Enhanced Raman Scattering (SERS) probes for the sensitive detection of Escherichia coliDH5α, as a model for Gram-negative bacteria. The nano-probes were easy to prepare through Au-S bonding. Under optimized conditions, the PATP-AuNPs surface with positive charge can bind with negatively charged E.coliDH5α via electrostatic adhesion, resulting in a quick color change from red to blue, and also a dramatic SERS signal enhancement from thousands of AuNPs aggregated on the surface of bacteria, which was utilized for both colorimetric and SERS detection of E.coliDH5α. For colorimetric analysis, it is the first time that the classical partial least square (PLS) regression was utilized to deal with the relationship between adsorption and E.coliDH5α concentrations. Excellent linear relationship was observed from 1.1 x 107 to 1.3 x 108 cfu mL-1 with the average relative error (ARE) of 5.430, which was more accurate than the traditional extinction ration method. When coupled with confocal Raman microscope, this PATP-AuNPs probes could be used to detection SERS signals produced from even one single bacterium. This bioassay is rapid, less expensive and convenient for bacteria detection and analysis. Therefore, PATP-AuNPs system as a novel, versatile, on-site and real-time Gram-negative bacteria sensor, would have a wide range of practical applications.
- Subjects
GOLD nanoparticles; GRAM-negative bacteria; COLORIMETRIC analysis; SERS spectroscopy; NANO-probe sensors; ELECTROSTATIC adhesion
- Publication
Journal of the Chinese Chemical Society, 2015, Vol 62, Issue 6, p521
- ISSN
0009-4536
- Publication type
Article
- DOI
10.1002/jccs.201400536