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- Title
Construction of suppression subtractive hybridization (SSH) library of copepod Pseudodiaptomous annandalei and its ferritin cDNA cloning and differential expression under nickel stress.
- Authors
Jiang Jie-lan; Wang Gui-zhong; Wu Li-sheng; Li Shao-jing
- Abstract
To study the molecular response mechanisms of copepod to nickel stress, a suppression subtractive hybridization (SSH) cDNA library of Pseudodiaptomous annandalei under nickel stress was constructed by using SSH technique, and a total of 140 clones were randomly picked from the growing colonies and identified by PCR. The recombinant rate of the library was 98. 6%, and the volume of the library was 1. 12×106 cfu. After the recombinant plasmids were sequenced, a partial cDNA fragment of ferritin was recognized based on BLAST searches in NCBI, with a size of 859 bp and continuously encoding 170 amino acid residues. The semi-quantitative PCR results showed that the ferritin cDNA under 24 h nickel stress was distinctly up-regulated. The successful construction of the SSH library and the obtaining of ferritin cDNA fragment would supply basis for the further study of the molecular response mechanisms of copepod to nickel stress.
- Subjects
MOLECULAR mechanisms of immunosuppression; IMMUNOLOGICAL tolerance; NUCLEIC acid hybridization; MOLECULAR biology; COPEPODA; REPRODUCTION; PHYSIOLOGICAL effects of nickel; ANTISENSE DNA; MOLECULAR cloning
- Publication
Yingyong Shengtai Xuebao, 2012, Vol 23, Issue 7, p1973
- ISSN
1001-9332
- Publication type
Article