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- Title
Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content.
- Authors
e Silva, Luis Felipe Lima; Techio, Vânia Helena; Resende, Luciane Vilela; Braz, Guilherme Tomaz; de Resende, Kátia Ferreira Marques; Samartini, Carolina Queiroz
- Abstract
The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.
- Subjects
BRAZIL; NUCLEAR DNA; VEGETABLES; MERISTEMS; HYDROXYQUINOLINE
- Publication
Crop Breeding & Applied Biotechnology, 2017, Vol 17, Issue 4, p320
- ISSN
1518-7853
- Publication type
Article
- DOI
10.1590/1984-70332017v17n4a49