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- Title
Statistical evaluation of the medium components for the production of high biomass, α-amylase and protease enzymes by Piriformospora indica using Plackett-Burman experimental design.
- Authors
Swetha, S.; Varma, Ajit; Padmavathi, T.
- Abstract
Piriformospora indica, a member of basidiomycota is an axenically cultivable endophytic fungus which exerts plant growth promoting effects on its host plant. P. indica is known to produce α-amylase and protease. Since the organism exhibits beneficial role in plant growth promotion, achieving high biomass is immensely essential. Hence to enable the commercial production, screening of medium components is a necessary step. The present paper investigates the screening of medium components using Plackett-Burman experimental design wherein the parameters such as α-amylase, protease and biomass have been examined. The parameters α-amylase, protease and biomass was found to vary from 0.25 to 0.45 mgmlmin, 0.1 to 0.15 mgml h and 0.8 to 22.6 g l, respectively, in 16 runs which demonstrates the strong influence of the medium components.
- Publication
3 Biotech, 2014, Vol 4, Issue 4, p439
- ISSN
2190-572X
- Publication type
Report
- DOI
10.1007/s13205-013-0168-7