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- Title
Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle.
- Authors
Pisani, Didier F.; Clement, Noémie; Loubat, Agnès; Plaisant, Magali; Sacconi, Sabrina; Kurzenne, Jean-Yves; Desnuelle, Claude; Dani, Christian; Dechesne, Claude A.
- Abstract
Skeletal muscle cells constitute a heterogeneous population that maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly, this population is also endowed with an adipogenic potential, even in humans, and intramuscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle-derived cells to decipher the cellular basis of the myoadipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myoadipogenic bipotent progenitors. These progenitors were isolated in the CD34 population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myoadipogenic CD56CD15 progenitors gives rise to myogenic CD56CD15 progenitors and adipogenic CD56CD15 progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations. STEM CELLS 2010;28:2182-2194
- Subjects
MYOBLASTS; HUMAN skeleton; MUSCLE cells; CELL populations; CELL membranes; GENE expression
- Publication
Stem Cells, 2010, Vol 28, Issue 12, p2182
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1002/stem.537