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- Title
Estimation of the infectious viral load required for transfusion-transmitted human T-lymphotropic virus type 1 infection ( TT- HTLV-1) and of the effectiveness of leukocyte reduction in preventing TT- HTLV-1.
- Authors
Sobata, R.; Matsumoto, C.; Uchida, S.; Suzuki, Y.; Satake, M.; Tadokoro, K.
- Abstract
Background and Objectives The risk of transfusion-transmitted human T-lymphotropic virus type 1 infection ( TT- HTLV-1) after prestorage leucocyte reduction ( LR) remains unknown, as the proviral load in the blood component that would cause TT- HTLV-1 is undetermined. On the basis of the distribution of HTLV-1 proviral load among HTLV-1-sero-positive blood donors, we attempted to estimate the proviral load for transfusion-related infectivity. We also discuss the effectiveness of LR in preventing TT- HTLV-1. Materials and Methods The HTLV-1 proviral load in 300 HTLV-1-sero-positive blood donors was determined by real-time polymerase chain reaction analysis. The proviral load required for transfusion-related infectivity was estimated using historical TT- HTLV-1 frequency data from a retrospective study on patients who had received blood from HTLV-1-sero-positive blood donors and the distribution pattern of HTLV-1 proviral load among blood donors. Results HTLV-1 proviral loads ranged between <0·01 and 25·0 copies per 100 leucocytes. Historical data showed TT- HTLV-1 frequency to be 80%. Assuming that 80% of the 300 sero-positive samples are infectious, it is estimated that the transfer of ≥9 × 104 cells containing the HTLV-1 provirus is required to establish TT- HTLV-1. Conclusion The residual number of HTLV-1-infected cells after LR is substantially lower than the viral load necessary for TT- HTLV-1. LR therefore appears to be effective in minimizing the incidence of TT- HTLV-1.
- Subjects
HTLV-I infections; BLOOD transfusion reaction; VIRAL load; LEUCOCYTES; POLYMERASE chain reaction; DISEASE incidence; PREVENTION
- Publication
Vox Sanguinis, 2015, Vol 109, Issue 2, p122
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12263