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- Title
In Vitro Inhibition of Hepatitis B Virus Replication by 2′,3′-Dideoxyguanosine, 2′,3′-Dideoxyinosine, and 3′-Azido-2′,3′-Dideoxythymidine in 2.2.15 (PR) Cells.
- Authors
Aoki-Sei, Shizuko; O'Brien, Mary C.; Ford, Harry; Fujii, Hiroyuki; Gilbert, Dennis A.; Cooney, David A.; Johns, David G.; Broder, Samuel; Mitsuya, Hiroaki
- Abstract
Hep G2-derived hepatoblastoma cells (2.2.15), which actively produce hepatitis B virus (HBY), were cultured in the presence of 2/,3′-dideoxyguanosine (ddG), 2′,3/-dideoxyinosine, or 3/-azido-2′,3/-dideoxythymidine (AZT). ddG was the most potent agent. It diminished viral replication by up to 95%, as assessed by the amount of episomal HBY DNA, without impairing cellular growth. AZT was the least effective against HBY. Northern blot analysis revealed no apparent difference in the pregenomic viral RNA profile, suggesting that these dideoxynucleosides suppress reverse transcription in the replicative cycle of HBY. The effect of varying the time of drug exposure showed that these agents can suppress HBY replication even when added late in culture. HBY replication in another 2.2.15 cell population of the same lineage was affected by ddG differently, which may enable the investigation of phenotypic or genetic alterations during culture. The present data suggest that some 2′,3′-dideoxynucleosides can exert a potent antiviral activity against HBY in vitro, at least under certain circumstances, although the data do not prove that any of these agents have utility in patients with hepatitis.
- Publication
Journal of Infectious Diseases, 1991, Vol 164, Issue 5, p843
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/164.5.843