We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Early Endosomal Retention of Murine Cytomegalovirus m06 Protein.
- Authors
Kučić, Natalia; Tomaš, Maja Ilić; Mahmutefendić, Hana; Blagojevic, Gordana; Lučin, Pero
- Abstract
Murine cytomegalovirus (MCMV) encodes several genes which products interact with MHC class I molecules (MHC-I) in the secretory pathway to evade antigen presentation. The product of MCMV-m06 gene associates with nascent MHC-I proteins and redirects them into lysosomes for degradation. This report will demonstrate that MCMV-m06 protein is retained in perinuclear early endosomes together with internalized MHC-I molecules. Since we were not able to capture the MCMV-m06 protein on the cell surface, our data suggests that the MCMV-m06 protein is transported from the Golgi into early endosomal intermediates prior to their maturation into multivesicular endosomes and lysosomes. In NIH 3T3 cells with the transfected m06 gene, the endosomal localization of the m06 protein was not observed, suggesting that another MCMV function remodels the route of m06 trafficking from the Golgi towards late endosomes.
- Subjects
CYTOMEGALOVIRUS diseases; LABORATORY mice; VIRAL proteins; LYSOSOMES; ENDOSOMES; GOLGI apparatus; MAJOR histocompatibility complex; ANTIGEN presentation
- Publication
Croatica Chemica Acta, 2012, Vol 85, Issue 2, p213
- ISSN
0011-1643
- Publication type
Article
- DOI
10.5562/cca1816