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- Title
Blotting protein complexes from native gels to electron microscopy grids.
- Authors
Knispel, Roland Wilhelm; Kofler, Christine; Boicu, Marius; Baumeister, Wolfgang; Nickell, Stephan
- Abstract
We report a simple and generic method for the direct transfer of protein complexes separated by native gel electrophoresis to electron microscopy grids. After transfer, sufficient material remains in the gel for identification and characterization by mass spectrometry. The method should facilitate higher-throughput single-particle analysis by substantially reducing the time needed for protein purification, as demonstrated for three complexes from Thermoplasma acidophilum.
- Subjects
GEL electrophoresis; ELECTRON microscopy; MASS spectrometry; PROTEIN analysis; MOLECULAR biology
- Publication
Nature Methods, 2012, Vol 9, Issue 2, p182
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1840