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- Title
A general life-death selection strategy for dissecting protein functions.
- Authors
Ear, Po Hien; Michnick, Stephen W.
- Abstract
Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
- Subjects
MOLECULAR biology; SACCHAROMYCES cerevisiae; CLONAL selection theory; PROTEINS; TRANSCRIPTION factors
- Publication
Nature Methods, 2009, Vol 6, Issue 11, p813
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1389