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- Title
Bright far-red fluorescent protein for whole-body imaging.
- Authors
Shcherbo, Dmitry; Merzlyak, Ekaterina M.; Chepurnykh, Tatiana V.; Fradkov, Arkady F.; Ermakova, Galina V.; Solovieva, Elena A.; Lukyanov, Konstantin A.; Bogdanova, Ekaterina A.; Zaraisky, Andrey G.; Lukyanov, Sergey; Chudakov, Dmitriy M.
- Abstract
For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.
- Subjects
PRESERVATION of organs, tissues, etc.; PROTEIN spectra; CRYOBIOLOGY; HISTOLOGY; ORGANS (Anatomy); BIOMOLECULES
- Publication
Nature Methods, 2007, Vol 4, Issue 9, p741
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1083