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- Title
DISTINGUISHING BETWEEN GLAUCOMYX VOLANS AND G. SABRINUS USING MICROSATELLITE ANALYSES.
- Authors
Viall, E. W.; Fellbaum, C. R.; Kiesow, A. M.
- Abstract
Northern flying squirrels, Glaucomys sabrinus, are difficult to distinguish from southern flying squirrels, Glaucomys volans, due to their morphological similarities. We used several microsatellite primers to genetically distinguish between suspected G. volans and G. sabrinus samples identified and collected in the field and determine the accuracy of key morphological characteristics used in field identification. Species specific microsatellite primers were identified using known specimens (G. sabrinus collected from the Black Hills and G. volans collected from central Wisconsin) of each species. G. volans and G. sabrinus were collected in central and northern Wisconsin in areas where these species overlap. Each specimen was identified in the field based on body size, skin pigmentation, weight, and hair color. DNA was extracted from all suspected G. volans and G. sabrinus specimens collected in the field. These samples were then amplified using the designated microsatellite primers. After genetically distinguishing each collected specimen, we compared the genetic data to the suspected identity of the species based on field identification. The results were statistically analyzed using JMP-IN software, and the current key morphological characteristics (body size, skin pigmentation, weight, and hair color) used in field identification were found to be somewhat effective.
- Subjects
WISCONSIN; NORTHERN flying squirrel; SCIURIDAE; MICROSATELLITE repeats; ANIMAL morphology; IDENTIFICATION of biological specimens; ANIMAL species; DNA; PHYSIOLOGY
- Publication
Proceedings of the South Dakota Academy of Science, 2009, Vol 88, p119
- ISSN
0096-378X
- Publication type
Article