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- Title
Green tea supplementation in high fat fed Sprague-Dawley rats has no effect on gene transcripts relating to muscle metabolism.
- Authors
Hinch, E.; Chen, N.; Mathai, M. L.; Carey, K. A.; Cameron-Smith, D.; Weisinger, H. S.; Sinclair, A. J.; Weisinger, R. S.; Lewandowski, P.
- Abstract
Background -- Functional food ingredients beneficial for weight regulation are of considerable importance given the marked increase in obesity experienced throughout the world. Green tea has previously been found to lower body fat and improve fat distribution in both human and animal models. Objectives -- This study aimed to investigate the impact of green tea, green tea catechins or black tea supplementation on rodent skeletal muscle gene transcripts essential for energy metabolism and lipid homeostasis. Design -- Sprague-Dawley rats were fed a high fat diet and supplemented at 100% of their fluid intake from 4 weeks of age, with water as a control (n=7), green tea (n=7), epigallocatechin-3-gallate (EGCG) (n=7) or black tea (n=7) for a period of 6 months. Following this, mRNA levels of genes important in energy metabolism including; cytochrome c oxidase 3 and 4 (COX 3 & 4) and lipid homeostasis including; fatty acid transferase (FAT/CD36), peroxisomal proliferators activated receptor alpha (PPAR-α), uncoupling protein-3 (UCP-3), peroxisome proliferators activatedreceptor gamma co activator 1alpha (PGC-1α) and pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) were measured by real-time PCR in quadriceps muscle samples. Outcomes -- There were no significance changes in mRNA expression of genes in groups supplemented with green tea, EGCG or black tea, this is despite a significant reduction in fat mass within the green tea and EGCG groups. Conclusion -- The supplements investigated did not elicit changes in the expression of genes essential for total oxidative and lipid metabolism in quadriceps muscle. This is despite the alterations in total adiposity evident following green tea and EGCG supplementation. Further analysis is required to evaluate the actions of green tea and catechins on other tissues, including adipocytes.
- Subjects
GREEN tea; SPRAGUE Dawley rats; GENETIC transcription; MUSCLE metabolism; TEA
- Publication
Asia Pacific Journal of Clinical Nutrition, 2005, Vol 14, pS76
- ISSN
0964-7058
- Publication type
Article