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- Title
Surface-enhanced Raman scattering as a tool to probe cytochrome P450-catalysed substrate oxidation.
- Authors
Bailo, Elena; Fruk, Ljiljana; Niemeyer, Christof M.; Deckert, Volker
- Abstract
Surface-enhanced Raman scattering was used as a spectroscopic tool to investigate the changes brought upon cytochrome P450BSß after fatty acid binding. Differences in the spectra of substrate-free and substrate-bound enzyme were observed indicating the potential for this method to be used in the screening of P450 substrates. In particular, the binding characteristics of myristic acid, an inherent substrate, and hydroxylauric acid, a product of fatty acid oxidation, towards P450BSß in the presence of H2O2 were investigated. Specific spectral changes could be assigned to changes in the heme environment only for myristic acid, indicating an occurrence of oxidation process characteristic for the enzymatic substrate.
- Subjects
RAMAN effect; CYTOCHROMES; BIOMOLECULES; PROTEINS; LIGAND binding (Biochemistry)
- Publication
Analytical & Bioanalytical Chemistry, 2009, Vol 394, Issue 7, p1797
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-009-2866-x