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- Title
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1SL GENOME OF Aegilops IN SUBUNIT longissima.
- Authors
Gengrui ZHU; Shunli WANG; Shoumin ZHEN; Xixi SHEN; PRODANOVIC, Slaven; Yueming YAN
- Abstract
Wheat related diploid species Ae. longissima (2n=2x=14, SlSl) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S¹-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S¹x-123, 1S¹x-129, 1S¹x1-136, 1S¹X2-136 and 1S¹x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S¹x-123, 2946 bp (979 aa) for 1S¹x-129, 2901 bp (965 aa) for 1S¹x1-136, 2982bp (991 aa) for 1S¹x2-136 and 2928 bp (974 aa) for 1SLX2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1Sl x-129 had an extra cysteine residue at the position 109 due to a TAT → TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
- Subjects
GLUTELINS; MOLECULAR phylogeny; PLANT germplasm; WHEAT quality; OPEN reading frames (Genetics); AEGILOPS
- Publication
Genetika (0534-0012), 2015, Vol 47, Issue 1, p185
- ISSN
0534-0012
- Publication type
Article
- DOI
10.2298/GENSR1501185Z